Biochemistry-pH Buffers

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Biochemistry-pH Buffers

Post by greenpak07 on Fri Jan 16, 2009 6:26 am

Hi,
I'm a bit confused as to how to solve this problem.

1. Calculate the amount of 0.5 M lactic acid/1.0M lactate you would need to make a 3.3M M100 mL solution with the pH of 4.80 and the pKa is 3. 9.

First you would use the equation .5M x (Y)= 3.3 M x (100mL)

that gives you 660 mL which is impossible since you have to make it 100mL?

Thanks,
Gina

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Re: Biochemistry-pH Buffers

Post by Alla on Sun Jan 18, 2009 10:56 pm

Hi Gina,

You are right; it is not possible to make 100ml of 3.3M solution out of 0.5M one. However, I would suggest you double check the problem - I think that
"3.3M M100 mL solution " was supposed to be 3.3 mM 100ml solution - then everything makes sense.

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